Abstract. Ovarian steroid hormones are frequently used in clinical practice. Postmenopausal replacement therapy and oral contraception are both based on the effects of the ovarian steroids. Mental side effects have often been reported, especially in relation to oral contraceptives. In that context it is of course of importance to collect knowledge about the influence ovarian steroid hormones have on the brain. The present review discusses the natural hormones estradiol and progesterone and some of their metabolites. Knowledge of the effects of synthetic estrogen and progestagen derivatives is very limited and will be mentioned only briefly.
The slope of the fitted lines between the LC-MS/MS and RIA measurements were all less than 1, indicating that testosterone, androstenedione, and 17-hydroxyprogesterone levels were lower in the LC-MS/MS compared to the RIAs ( Figure 2 ). The Bland-Altman plots demonstrated that the percent difference between the RIAs and the LC-MS/MS ranged from −58 to 186% ( Figure 3 ). The average percent difference in levels between the RIAs and LC-MS/MS was lowest for testosterone (31±38%; mean±SD), and highest for progesterone measured in the follicular phase (89±54%), while androstenedione and 17-hydroxyprogesterone had similar average percent differences (60±22 and 63±50%, respectively). There was no difference in the percent difference in RIA and LC-MS/MS results in women with PCOS compared to controls for testosterone, androstenedione and 17-hydroxyprogesterone (all p>). Progesterone levels were more variable in women with PCOS (106±41%) than in controls (71±60%; p<).